影像服务

The 图 组织学和影像学核心 offers services and training related to image analysis and image analysis software, to guide investigators in choosing the best methods for presenting their data.

• Thorlabs Bergamo II multi-photon microscope equipped with SpectraPhysics Insight X3 dual emission laser source
  • 20倍，60倍水浸镜头
  • Galvo-galvo and resonant-galvo scan paths for imaging and stimulation
• Olympus VS200 slide scanning microscope
  • Fully automated and enclosed with 6-slide sample holder; DAPI, 绿色荧光蛋白, TRITC, Cy5 filter cubes; Orca Fusion 16-bit sCMOS camera and Excelitas LED excitation source
• Leica TCS SP5 confocal laser scanning microscope
  • 10倍计划流量/0.3、20倍平面流量/0.7、40倍油计载量/1.3、63 ×油计载量/1.4,63 x水计划载脂蛋白/1.2
  • 2个pmt, 1个HyD检测器
  • 405, 415, 458, 476, 488, 514, 561, 633 nm laser lines
  • Tokai Hit stage top incubation system
  • 用于快速成像的共振扫描仪
• Keyence BZ-X710 inverted widefield digital microscope; DAPI, 绿色荧光蛋白, TRITC, Cy5 filter cubes; 4x plan fluor/0.15、10倍平面厚度/0.3、20 ×平面层ELWD/0.45、20倍计划载比/0.7、40 ×平面楼层/0.5、40 ×平面楼层/0.75、100 ×油计划氟/1.3
  • 12位单色/彩色CCD
  • XY stitching, Z-stack, and multi-point acquisition (can be combined)
  • BZ-X analyzer software for image stitching, quantification, and analysis
• Leica DM2500 upright widefield microscope with automated stage; DAPI, Cy2, Cy3, Cy5 filter cubes; 5x plan fluo/0.15、10倍平面流量/0.3、20倍平面流量/0.5、40倍平面流量/0.75、63 ×油计载量/1.4
  • Stereologer unbiased image analysis software
  • 徕卡DFC 365 FX 16位.4mp单色CCD
  • Imi-Tech Nile2 5 MP彩色CCD
• Nikon E800 upright widefield microscope (x2); DAPI, Cy2, Cy3 filter cubes; 4x plan fluo/0.14、10倍平面流量/0.3,40 x平面流量/0.75、100倍油计划氟/1.3
  • 诊断仪器RT KE点7.2彩色拼接CCD
• Nikon E400 dual-headed brightfield microscope
  • 2 x计划/ 0.06,4x plan/0.1、10x plan/0.25、20倍平面/0.4、40倍平面/0.65
  • ImagingPlanet MPX 3.3mp彩色CCD
• Leica MZ6 dual-headed stereomicroscope
  • 4.0 x变焦
  • Jenoptik ProgRes CFcool 14-bit cooled CCD

Cypher is the first commercial AFM to take advantage of the high bandwidth and low noise performance of smaller cantilevers and is the only commercial AFM to routinely resolve atomic point defects and the DNA helix. 现在, Asylum 研究 introduces the Cypher ES, which adds full environmental control to the Cypher family. The heart of the Cypher ES is based around a cell constructed of inert materials to enclose and control the sample environment. The modular design allows customization for measurements in nearly any environment.

• 密封细胞中的气体/流体灌注
  • Multiple ports for flow-through of gas and/or fluid
  • Perfusion may be performed in a droplet to minimize sample volume (<20µL) and cell cleaning
  • Simple gravity driven perfusion — no pumps are required
• 集成温度控制
  • Modular sample stages enable precise temperature control in several temperature ranges
  • No external boxes, additional electronics, or cooling pumps are required
• 广泛的化学相容性
  • Ensuring chemical compatibility even in the harshest environments, cells are constructed with inert materials:
  • 熔融石英
  • Perfluoroelastomer (FFKM)
  • 聚醚醚酮(PEEK)
  • Suitable for solvents, acids, bases, buffers, inert gases
  • 密码AFM规范
• 头/光学杠杆
  • DC Detector Noise: <5pm
  • AC Detector Noise: <25fm/√Hz above 100kHz
  • 光电二极管带宽:DC至7MHz
  • Light Source: User selectable/exchangeable. Options include superluminescent diodes and laser diodes. 波长固定在850nm.
  • Spot Size: User selectable/exchangeable. Focused spots range in size from 10x30µm down to 3x9µm.
  • Controls: Focused spot positioning and photodiode centering controls are motorized and fully controllable from the software.

For general questions or to submit an 影像服务申请表格, email 组织学和影像学核心 Manager 彼得Caradonna at pcaradonna@dektinary.com.

教师和专业人员